Instrument: Illumina MiSeq
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA and small RNA fractions (≤ 200 nt) were extracted from the leaves inoculated by SMV-L, SMV-G7 and Mock using a mirVana miRNA isolation kit (Ambion) and the Poly (A)+ RNA was isolated using the NucleoTrap@ mRNA purification kits (Machery-Nagel) according to the instructions of the supplier. RNA-seq and sRNA-seq libraries were prepared following the manufacturer's instructions using Illumina TruSeq Stranded Total RNA Sample Prep Kit (Cat#RS-122-2501) and TruSeq Small RNA Sample Prep Kit (Cat#RS-200-0012),respectively. Degradome-seq libraries were constructed as previously described using Illumina TruSeq Small RNA Sample Prep Kit (Cat#RS-200-0024)(German, M. A. et al., Nature Biotechology, 2008; German et al., Nature Protocols, 2009; Zhai et al., 2014).